Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

Author:

Amin Imran1,Jabbar Tania12,Niazi Fawad1,Akhtar Muhammad Saeed3

Affiliation:

1. PCR Laboratory, Punjab Institute of Nuclear Medicine (PINUM), Faisalabad 2019, Pakistan

2. Department of Clinical Pathology, Punjab Institute of Nuclear Medicine (PINUM), Faisalabad 2019, Pakistan

3. Nuclear Medicine Department, Punjab Institute of Nuclear Medicine (PINUM), Faisalabad 2019, Pakistan

Abstract

A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1×(106, 105, 104, and 103) IU mL−1. Comparison of test results obtained by two extraction methods showed a good correlation (r=0.95,n=30) with detection limit of 102 IU mL−1. The semiautomated vacuum filtration based protocol demonstrated high throughput: 35 minutes for the extraction of a batch of 30 samples (150 µL each) with reduced labor, time, waste, and cost. Performance characteristics of semiautomated system make it suitable for use in diagnostic purpose and viral load determinations.

Publisher

Hindawi Limited

Subject

Analytical Chemistry

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