Affiliation:
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Department of Traditional Chinese Medicine, Henan Province People’s Hospital, Zhengzhou 221000, China
Abstract
Pterocephalus hookeriis a widely applied Tibetan medicinal prescription for treatment of diseases such as flu, rheumatoid arthritis, and enteritis in China. It has been reported thatPterocephalus hookerihas anti-inflammatory and analgesic actions. However, the antitumor activity ofPterocephalus hookeriremains unknown. In the present study, we demonstrate that n-butanol extracts ofPterocephalus hookeri(YSC-ZDC) has a strong antitumor activity against hepatoma carcinoma cellin vitroandin vivo. YSC-ZDC inhibited proliferation of all cancer cell lines and significantly inhibited Hep3B cells proliferation in a dose- and time-dependant manner. Transmission electron microscopy, hoechst 33258 staining, and flow cytometry analysis revealed that YSC-ZDC induced apoptosis in Hep3B cells. YSC-ZDC treatment dramatically inhibited PDK1 and Akt phosphorylation in Hep3B cells. Moreover, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression. In addition, YSC-ZDC inhibited growth hepatoma xenograftsin vivowith no effect on body weight and spleen index. Consistent with resultsin vitro, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression in tumor tissue. Taken together, this study shows YSC-ZDC with an antitumor activity bothin vitroandin vivo. Its mechanism underlying is related to blocking of the Akt pathway and regulation of Bcl-2 family proteins expression.
Funder
Qianbowen Teacher Studio Fund
Subject
Complementary and alternative medicine
Cited by
6 articles.
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