Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection ofCampylobacter jejuniin Live Poultry

Abstract

The preparation of antibodies-conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) was developed to detectCampylobacter jejunicells under a fluorescence microscope. The particles prepared by sol-gel microemulsion techniques have a round shape with an average size of 43 ± 4 nm. They were highly photo stable and could emit strong orange fluorescent for 60 min. Both amine- and carboxyl-functionalized properties were evident from FTIR and FT Raman spectra. The FDS-NPs conjugated with antibodies againstC. jejuniwere well dispersed in PBS solution at 20 mM of NaCl. The conjugation with monoclonal antibodies againstC. jejuniwas successful. The direct observation of the antibodies-conjugated FDS-NPs- that boundsC. jejuniwith Petroff Hausser counting chamber at 40x was clear. The different focus lengths clearly separated bound and unbound FDS-NPs under the microscope. We successfully synthesis the bio-conjugated dye doped silica nanoparticles forC. jejunithat are easy to use and giving clear detection in due time.