Noninvasive Superoxide Monitoring ofIn VitroNeuronal Differentiation Using a Cell-Based Biosensor-Reference-Cited by-同舟云学术

Noninvasive Superoxide Monitoring ofIn VitroNeuronal Differentiation Using a Cell-Based Biosensor

Published:2015 Issue: Volume:2015 Page:1-6
ISSN:1687-725X
Container-title:Journal of Sensors
language:en
Short-container-title:Journal of Sensors

Author:

Moschopoulou Georgia¹,Kintzios Spyridon¹

Affiliation:

1. Faculty of Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece

Abstract

Membrane-engineered cells bearing superoxide dismutase (SOD) molecules on their surface offer the capability of ultrarapid and ultrasensitive detection of the superoxide anion (O2-) through the measurement of changes of their cell membrane potential. We herewith report the application of this technology for the noninvasive determination of superoxide levels during thein vitrodifferentiation of PC12 cells. We were able to detect changes inO2-accumulation in the culture medium, which were closely associated with the progress of neuronal differentiation.

Publisher

Hindawi Limited

Subject

Electrical and Electronic Engineering,Instrumentation,Control and Systems Engineering

Link

http://downloads.hindawi.com/journals/js/2015/768352.pdf

Reference25 articles.

1. Energetics, epigenetics, mitochondrial genetics

2. Modulation of neuronal stem cell differentiation by hypoxia and reactive oxygen species

3. Semaphorin 3A Stimulates Neurite Extension and Regulates Gene Expression in PC12 Cells

4. Docosahexaenoic acid induces ERK1/2 activation and neuritogenesis via intracellular reactive oxygen species production in human neuroblastoma SH-SY5Y cells

5. Moderate superoxide production is an early promoter of mitochondrial biogenesis in differentiating N2a neuroblastoma cells

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