Differences in the Antigens ofHelicobacter pyloriStrains Influence on the Innate Immune Response in theIn VitroExperiments

Abstract

The immune response toHelicobacter pyloriimportantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. Strains ofH. pyloriwere gathered from 14 patients who failed to eradicateH. pyloriinfection with antibiotics—therapy resistant strains (TRS)—or from patients who were able to eradicateH. pyloriinfection—therapy susceptible strains (TSS). The THP-1 cells were stimulated withH. pyloriantigens. Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer. TSSH. pyloriantigens increased the proportion of cathepsin X positive cells compared to TRSH. pyloriantigens. TSSH. pyloriantigens induced higher secretion of IL-12 and IL-6 compared to TRSH. pyloriantigens (P<0.001; 0.02). Polymyxin B, a lipid A inhibitor, lowered the secretion of IL-12 and IL-6 in TRS and TSS. We demonstrated aH. pyloristrain-dependent cathepsin X and cytokine expression that can be associated withH. pyloriresistance to eradication due to lack of effective immune response. Differences in lipid A ofH. pylorimight have an influence on the insufficient immune response, especially on phagocytosis.