Biochemical Characterization, Action on Macrophages, and Superoxide Anion Production of Four Basic Phospholipases A2from PanamanianBothrops asperSnake Venom

Author:

Rueda Aristides Quintero123,Rodríguez Isela González1,Arantes Eliane C.4,Setúbal Sulamita S.5,Calderon Leonardo de A.5,Zuliani Juliana P.5,Stábeli Rodrigo G.5,Soares Andreimar M.5

Affiliation:

1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, FCFRP-USP, Ribeirão Preto, SP, Brazil

2. Departamento de Química, Facultad de Ciencias Naturales y Exactas, Universidad Autónoma de Chiriquí, UNACHI, Panama

3. Departamento de Laboratorio Clínico, Complejo Hospitalario Metropolitano, Caja de Seguro Social, CHM-CSS, Panama

4. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, FCFRP-USP, Ribeirão Preto, SP, Brazil

5. Centro de Estudos de Biomoléculas Aplicadas a Saúde-CEBio, Fundação Oswaldo Cruz, FIOCRUZ Rondônia e Núcleo de Saúde, Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil

Abstract

Bothrops asper(Squamata: Viperidae) is the most important venomous snake in Central America, being responsible for the majority of snakebite accidents. Four basic PLA2s (pMTX-I to -IV) were purified from crude venom by a single-step chromatography using a CM-Sepharose ion-exchange column (1.5 × 15 cm). Analysis of the N-terminal sequence demonstrated that pMTX-I and III belong to the catalytically active Asp49 phospholipase A2subclass, whereas pMTX-II and IV belong to the enzymatically inactive Lys49 PLA2s-like subclass. The PLA2s isolated from PanamaBothrops aspervenom (pMTX-I, II, III, and IV) are able to induce myotoxic activity, inflammatory reaction mainly leukocyte migration to the muscle, and induce J774A.1 macrophages activation to start phagocytic activity and superoxide production.

Funder

Organization of American States

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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