Preclinical Evaluation of Antiurolithiatic Activity ofViburnum opulusL. on Sodium Oxalate-Induced Urolithiasis Rat Model

Abstract

The aim of the present research is to evaluate the antiurolithiatic effect of the various extracts prepared from the fruits ofViburnum opulusL., in regard to its ethnobotanical record. To induce urolithiasis, 70 mg/kg sodium oxalate was injected to the rats which were housed individually in metabolic cages. The test materials were applied during 7 days. Biochemical (urine and serum parameters), histopathological and antioxidant (TBARs, TSH and GSH) assays were conducted. The urine samples were examined by light microscope for the determination of the calcium oxalate crystals. Lyophilized juice ofV. opulus(LJVO) and lyophilized commercial juice ofV. opulus(LCJVO) exerted potential antiurolithiatic activity which was attributed to its diuretic effect along with the inhibitory action on the oxalate levels and free radical production. We also determined the chlorogenic acid content of the LJVO by high-performance liquid chromatography (HPLC). Chlorogenic acid was determined by using Supelcosil LC-18 (250×4.6 mm, 5 µm) column and acetonitrile: water: 0.2%o-phosphoric acid as a mobile phase. The chlorogenic acid content ofV. opuluswas found to be 0.3227 mg/mL in fruit juice. The results obtained in this study have provided a scientific evidence for the traditional usage ofV. opuluson passing kidney stones in Turkish folk medicine.