Characterisation of Potential Antidiabetic-Related Proteins fromPleurotus pulmonarius(Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOF/TOF Mass Spectrometry

Abstract

Pleurotus pulmonariushas been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment. This study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions fromP. pulmonariusbasidiocarps. Preliminary results demonstrated that 30% (NH4)2SO4precipitated fraction (F30) inhibitedSaccharomyces cerevisiaeα-glucosidase activity (24.18%), and 100% (NH4)2SO4precipitated fraction (F100) inhibited porcine pancreatic α-amylase activity (41.80%). Following RP-HPLC purification, peak 3 from F30 fraction demonstrated inhibition towards α-glucosidase at the same time with meagre inhibition towards α-amylase activity. Characterisation of proteins using MALDI-TOF/TOF MS demonstrated the presence of four different proteins, which could be implicated in the regulation of blood glucose level via various mechanisms. Therefore, this study revealed the presence of four antidiabetic-related proteins which are profilin-like protein, glyceraldehyde-3-phosphate dehydrogenase-like protein, trehalose phosphorylase-like (TP-like) protein, and catalase-like protein. Hence,P. pulmonariusbasidiocarps have high potential in lowering blood glucose level, reducing insulin resistance and vascular complications.