Parishin fromGastrodia elataExtends the Lifespan of Yeast via Regulation of Sir2/Uth1/TOR Signaling Pathway

Abstract

Parishin is a phenolic glucoside isolated fromGastrodia elata, which is an important traditional Chinese medicine; this glucoside significantly extended the replicative lifespan of K6001 yeast at 3, 10, and 30 μM. To clarify its mechanism of action, assessment of oxidative stress resistance, superoxide dismutase (SOD) activity, malondialdehyde (MDA), and reactive oxygen species (ROS) assays, replicative lifespans ofsod1,sod2,uth1, andskn7yeast mutants, and real-time quantitative PCR (RT-PCR) analysis were conducted. The significant increase of cell survival rate in oxidative stress condition was observed in parishin-treated groups. Silent information regulator 2 (Sir2) gene expression and SOD activity were significantly increased after treating parishin in normal condition. Meanwhile, the levels of ROS and MDA in yeast were significantly decreased. The replicative lifespans ofsod1,sod2,uth1,andskn7mutants of K6001 yeast were not affected by parishin. We also found that parishin could decrease the gene expression ofTORC1, ribosomal protein S26A (RPS26A), and ribosomal protein L9A (RPL9A) in the target of rapamycin (TOR) signaling pathway. Gene expression levels ofRPS26AandRPL9Ainuth1, as well as inuth1,sir2double mutants, were significantly lower than those of the control group. Besides,TORC1gene expression inuth1mutant of K6001 yeast was inhibited significantly. These results suggested that parishin exhibited antiaging effects via regulation of Sir2/Uth1/TOR signaling pathway.