The Antimicrobial and Wound Healing Potential ofOpuntia ficus indica L. inermisExtracted Oil from Tunisia

Abstract

Introduction.Opuntia ficus indica L. inermis(OFI) is used in traditional medicine pharmacopeia for its richness in natural bioactive compounds. It has been proven to be effective in the improvement of the healing of laser-induced skin burns. The aim of the present study was to investigate the wound healing effect of OFI extracted oil on full-thickness skin wound.Materials and Methods. The OFI seeds were firstly isolated from mature prickly pears, washed, dried, and then cold-pressed. The antimicrobial activities of OFI seed oil were estimatedin vitroagainst bacteria, yeast, and fungi. Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were calculated. Skin wound healing was investigated using an excisional wound healing model in rats. The skin wounds of three experimental groups of rats were topically treated once/day with saline solution (control group), 0.15 mg/mm2of a reference drug Esth’Elle Pharma Cicaplaie cream (reference group), and 0.6 μl/mm2of OFI seed oil (OFI oil group). The healing process was monitored daily and the percentage of wound contraction was calculated. A histological study was carried on skin biopsies.Results. The extracted oil has shown an interesting antimicrobial effect onEnterobacter cloacae, antiyeast effect againstCandida parapsilosisandCandida sake, and antifungal activity against three opportunistic cutaneous molds (Penicillium, Aspergillus, and Fusarium). Moreover, OFI oil has shown a good wound healing effect. It prevents cutaneous infections and reduces the reepithelialization phase.Conclusion. OFI extracted oil hasin vitroantimicrobial/fungal properties andin vivowound healing activity. It seems to be efficient in the treatment of cutaneous infections and the promoting of the scarring process.