Antioxidative Role of Hatikana (Leea macrophyllaRoxb.) Partially Improves the Hepatic Damage Induced by CCl4in Wistar Albino Rats

Abstract

This research investigated the protective role ofLeea macrophyllaextract on CCl4-induced acute liver injury in rats. Different fractions ofLeea macrophylla(Roxb.) crude extract were subjected to analysis for antioxidative effects. Rats were randomly divided into four groups as normal control, hepatic control, and reference control (silymarin) group and treatment group. Evaluations were made for the effects of the fractions on serum enzymes and biochemical parameters of CCl4-induced albino rat. Histopathological screening was also performed to evaluate the changes of liver tissue before and after treatment. Different fractions ofLeea macrophyllashowed very potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, FeCl3reducing effect, superoxide scavenging effect, and iron chelating effect. Carbon tetrachloride induction increased the level of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and other biochemical parameters such as lipid profiles, total protein, and CK-MB. In contrast, treatment ofLeea macrophyllareduced the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities as well as biochemical parameters activities.L. macrophyllapartially restored the lipid profiles, total protein, and CK-MB. Histopathology showed the treated liver towards restoration. Results evidenced thatL. macrophyllacan be prospective source of hepatic management in liver injury.