Estimation of Bioactive Compound, Maslinic Acid by HPTLC, and Evaluation of Hepatoprotective Activity on Fruit Pulp ofZiziphus jujubaMill. Cultivars in India

Abstract

Fruits ofZiziphus jujubaMill. (family: Rhamnaceae), known as Indian jujube or “Ber,” are of potential nutritional and medicinal value. The objectives of the present study were to estimate bioactive compound maslinic acid by HPTLC method and to evaluatein vitroantioxidant and hepatoprotective activity of eight cultivars of Indian jujube. Maslinic acid and the fruit pulp of various cultivars of Indian jujube, namely,Gola,Sannur,Umaran,Mehrun, andChhuhara, exhibited significantly high antioxidant and hepatoprotective activity. HPTLC-densitometric method was developed for quantification of maslinic acid from fruits of Indian jujube cultivars. The trend of occurrence of maslinic acid in fruits pulp extracts was as follows:Gola>Sannur>Umaran>Mehrun>Chhuhara> Wild >Kadaka>Apple. A significant correlation was shown by maslinic acid content and prevention of oxidative stress induced by CCl4in liver slice culture cell treated with extract. Maslinic acid along with its other phytoconstituents like phenols, flavonoids, and ascorbic acid may act as a possible therapeutic agent for preventing hepatotoxicity caused by oxidative stress generated due to the prooxidants like CCl4. This is the first report of fruit pulp extracts ofZ. jujubecultivars in India and maslinic acid preventing CCl4induced damage in liver slice culture cell of mice.