Cytotoxic Effect of flavonoids extracted from Conocarpus erectus leaves on SKG cell

Abstract

The present study was conducted to investigate the cytotoxic activity flavonoids extracted from Conocarpus erectus L. leaves using ethyl acetate and chloroform, the results of the extracted flavonoids were detected by HPLC. The evaluation of its cytotoxic effect on cancerous SKG cell line Human Esophageal Cancer and non-cancerous REF (rat embryonic fibroblast) using MTT assay. HPLC analysis of both extracts showed 6 out of 10 flavonoids matched the standard peaks which were (Catechin, Rutin, Myrecetin, Quercetin, Apigenine and Kaempferol). The cytotoxicity of the ethyl acetate extract was greater than that of the chloroform extract against the SKG cell line, and the cytotoxic effect of both extracts increased with increasing concentration. At 200 μl/ml the cytotoxicity of the ethyl acetate and chloroform extracts were 71% and 64.6%, respectively, while the cytotoxicity of the extracts on REF cells was 10.5% and 11.2%. This implies that C.erectus extract has little negative effects on normal cells and is thus a safe and promising anticancer drug candidate.