Affiliation:
1. Tver State Technical University
2. All-Russian Research Institute of Reclaimed Lands
Abstract
The paper presents experimental results on an effective method of using a complex of redox enzymes of the oxidoreductase class: glucooxidase (CF 1.1.3.4) and peroxidase (CF 1.11.1.7). Literature data confirming an increase in the potential of the redox reaction with the additional introduction of peroxidase, which decomposes hydrogen peroxide during the reaction used in the production of biofuel elements, are presented. The enzyme complex was included in polymer matrices of polyvinylpyrrolidone and acetylcellulose, which have a large number of functional groups, such as amide, hydroxyl, and carboxyl, capable of forming strong covalent bonds with enzymes, as well as a high sorption capacity to proteins. The paper presents a comparative characteristic of the activities of biopolymer complexes. The data of the Fourier-IR spectroscopy prove the formation of strong covalent bonds between the functional groups of enzymes and the matrix used.
Funder
Russian Foundation for Basic Research
Publisher
Publishing Center Science and Practice
Cited by
2 articles.
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