Etodolac Enhances the Radiosensitivity of Irradiated HT-29 Human Colorectal Cancer Cells-Reference-Cited by-同舟云学术

Etodolac Enhances the Radiosensitivity of Irradiated HT-29 Human Colorectal Cancer Cells

Published:2022-09 Issue:3 Volume:15 Page:242-248
ISSN:1874-4710
Container-title:Current Radiopharmaceuticals
language:en
Short-container-title:CRP

Author:

Alvandi Maryam¹^ORCID,Shaghaghi Zahra²³^ORCID,Polgardani Nasim Zarei⁴,Abbasi Sahar⁵,Albooyeh Hajar⁶,Dastranj Leila⁷,Farzipour Soghra⁸⁹

Affiliation:

1. Department of Nuclear Medicine and Molecular Imaging, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

3. Department of Nuclear Medicine and Molecular Imaging, Clinical Development Research Unit of Farshchian Heart Center, Hamadan University of Medical Sciences, Hamadan, Iran

4. Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Science, Sari, Iran

5. Department of Radiology, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6. Department of Nuclear Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

7. Department of Physics, Hakim Sabzevari University, Sabzevar, Iran

8. Department of Cardiology, Cardiovascular Diseases Research Center, Heshmat Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

9. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran

Abstract

Background: Radioresistance is found to be the main therapeutic restriction in colorectal radiation therapy. The aim of this study was to investigate the synergistic effect of Etodolac (ET) and ionizing radiation on human colorectal cancer cells. Materials & Methods: Pretreated HT-29 cells with ET were exposed to ionizing radiation. The radiosensitizing effect of ET was evaluated using MTT, flow cytometry, and clonogenic assay. The amount of nitrite oxide (NO) in irradiated cells was also measured with the Griess reagent. Results: The present study found that pretreatment of HT-29 cells with ET decreases their survival and colony formation. Higher concentrations of ET cause total apoptosis and an increase in NO levels in irradiated cells. Conclusion: Applying ET in a concentration-dependent manner had an incremental effect on the amount of apoptosis and cell death induced by radiation.

Publisher

Bentham Science Publishers Ltd.

Subject

Pharmacology,Radiology, Nuclear Medicine and imaging

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