Solvent bar microextraction combined with HPLC-DAD for simultaneous determination of diuretics in human urine and plasma samples

Abstract

Background: A simple and powerful microextraction procedure, the solvent bar microextraction (SBME), was used for the simultaneous determination of two diuretics, furosemide and spironolactone in human urine and plasma samples, using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Methods: The appropriate amount (2 µL) of 1-octanol as an organic solvent confined within (2.5 cm) of a porous hollow fiber micro-tube, sealed at both ends was used for this procedure. The conditions for the SBME were optimized in water and the analytical performance were examined in spiked human urine and plasma samples. Results: The optimized method exhibited good linearity (R2 > 0.997) over the studied range of higher than 33 to 104 µg L-1 for furosemide and spironolactone in urine and plasma samples, illustrating a satisfactory precision level with RSD values between 2.1% and 9.1%. Discussion: The values of the limits of detection were found to be in the range of 6.39 to 9.67 µg L-1, and extraction recovery˃ 58.8% for both diuretics in urine and plasma samples. The applicability and effectiveness of the proposed method for the determination of furosemide and spironolactone in patient urine samples were tested. Conclusion: In comparison with reference methods, the attained results demonstrated that SBME combined with HPLC-DAD was proved to be simple, inexpensive, and promising analytical technology for the simultaneous determination of furosemide and spironolactone in urine and plasma samples.