Affiliation:
1. Vaccine Research Center, Iran University of Medical Sciences, Tehran, Iran
2. Iran Ministry of Health and Medical
Education, Deputy Ministry for Education, Tehran, Iran
3. Department of Radiopharmacy, Faculty of Pharmacy, Tehran
University of Medical Sciences, Tehran, Iran
Abstract
Abstract:
Conjugation of polyethylene glycol (PEGylation) to blood proteins and cells has
emerged as a successful approach to address some of the issues attributed to the storage of blood
products, including their short half-life and instability. In this regard, this review study aims to
compare the influence of different PEGylation strategies on the quality of several blood products
like red blood cells (RBCs), platelets, plasma proteins, i.e., albumin, coagulation factor VIII, and
antibodies. The results indicated that conjugating succinimidyl carbonate methoxyPEG
(SCmPEG) to platelets could improve blood transfusion safety by preventing these cells from
being attached to low-load hidden bacteria in blood products. Moreover, coating of 20 kD succin- imidyl valerate (SVA)-mPEG to RBCs was able to extend the half-life and stability of these cells
during storage, as well as immune camouflage their surface antigens to prevent alloimmunisation.
As regards albumin products, PEGylation improved the albumin stability, especially during sterilization,
and there was a relationship between the molecular weight (MW) of PEG molecules and
the biological half-life of the conjugate. Although coating antibodies with short-chain PEG molecules
could enhance their stabilities, these modified proteins were cleared from the blood faster.
Also, branched PEG molecules enhanced the retention and shielding of the fragmented and
bispecific antibodies. Overall, the results of this literature review indicate that PEGylation can be
considered a useful tool for enhancing the stability and storage of blood components.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Biotechnology
Cited by
3 articles.
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