Affiliation:
1. Department of Food Science and Chemistry, University of the Philippines Mindanao, Davao, Philippines
Abstract
Introduction:
Silver nanoparticles (AgNPs) were synthesized using mangosteen pericarp ethanolic
extract (MPEE) as a source of bioreductants and their antimicrobial activity against common foodborne
pathogens was evaluated.
Methods:
Characterization of MPEE was conducted using phytochemical screening, total phenolic content
analysis, and DPPH (antioxidant) assay. Synthesis AgNPs and optimization studies were monitored
using UV-Vis spectrophotometry. Transmission electron microscopy was used to characterize the AgNPs,
and resazurin microtiter assay was used for antimicrobial testing.
Results:
Alkaloids, flavonoids, saponins, quinones, anthraquinones, and tannins were confirmed present
in the extract. TPC and IC50 of MPEE were 0.192 mg GAE/mg extract and 0.277 mg/mL, respectively. A
surface plasmon resonance (SPR) peak within 450-403 nm confirmed the formation of AgNPs. At pH 7,
the optimum reaction conditions were 45°C and 3 h. Meanwhile, at pH 9, the optimum reaction conditions
were 27°C and 0.5 h. The sizes of nanoparticles synthesized at pH 7 and pH 9 were 13-35 nm and 7-
38 nm, respectively. The minimum inhibitory concentration (MIC90) of AgNPs produced at pH 7 were
1.45, 2.81, and 2.93 ug/mL for S. aureus, E.coli, and B. cereus, respectively. For AgNPs synthesized at
pH 9, the MIC90 were 2.93, 3.02, and 5.24 ug/mL, for the same microorganisms, respectively.
Conclusion:
MPEE was able to successfully synthesize AgNPs. Compared to chloramphenicol, AgNPs
exhibited better antimicrobial activity, which can address the growing concern of drug resistance in certain
pathogenic microorganisms. Furthermore, the use of MPEE provides a green and sustainable alternative
to synthesizing AgNPs.
Publisher
Bentham Science Publishers Ltd.
Subject
General Engineering,General Materials Science
Reference64 articles.
1. Benelmekki M.; An introduction to nanoparticles and nanotechnology. Designing Hybrid Nanoparticles 2015,1-14
2. Khan I.; Saeed K.; Khan I.; Nanoparticles: Properties, applications and toxicities. Arab J Chem 2019,12(7),908-931
3. Nasrollahzadeh M.; Sajadi S.; Sajjadi M.; Issaabadi Z.; An Introduction to Green Nanotechnology 2019,1-27
4. Fu P.P.; Xia Q.; Hwang H-M.; Ray P.C.; Yu H.; Mechanisms of nanotoxicity: Generation of reactive oxygen species. Yao Wu Shi Pin Fen Xi 2014,22(1),64-75
5. Nalwade A.R.; Jadhav A.; Biosynthesis of silver nanoparticles using leaf extract of Daturaalba Nees. and evaluation of their antibacterial activity. Arch Appl Sci Res 2013,5,45-49
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献