Author:
Srisatjaluk Ratchapin Laovanitch,Kaewjiaranai Thanawat,Wongsirichat Natthamet
Abstract
Background:Antimicrobial properties of some anesthetic agents have been reported which may be useful for infection control. Topical lidociane (10%) showed some extent of antimicrobial activity on oral microflora.Objective:This study aimed to determine whether the antimicrobial efficiency of topical lidocaine can be enhanced by adding chlorhexidine.Methods:The Lidocaine-Chlorhexidine (LD-CHX) preparation was prepared by mixing 2.0% CHX and 10% LD with various ratios. The anesthetic efficiency was tested on the oral mucosa of 26 volunteers by Pin Prick Test. Pain scores were recorded using the Numerical Rating Scale (NRS). Antimicrobial effects of 10% LD, 0.2% CHX, and LD-CHX preparations were evaluated.In vitrostudy was conducted against seven standard bacterial strains. Forin vivostudy, oral biofilms of 26 volunteers were collected by imprint technique using filter papers. Bacterial growth from the samples after applying the test solutions was compared to the control. Antimicrobial efficiency was expressed as microbial reduction scores from 4-0 (highest to lowest).Results:The LD-CHX preparations that had comparable anesthetic efficiency to 10% LD were those containing 9% and 8% LD. These LD-CHX preparations showed microbial reduction scores of 3 and 4 on seven bacterial strains and oral biofilms.Conclusion:This study showed the antimicrobial and anesthetic effectiveness of new lidocaine-chlorhexidine preparations bothin vitroandin vivo.We also confirmed the effectiveness of infection control protocol in oral surgery using CHX mouthwash prior to topical LD.
Publisher
Bentham Science Publishers Ltd.
Reference26 articles.
1. Ogle OE, Mahjoubi G. Local anesthesia: Agents, techniques, and complications. Dent Clin North Am 2012; 56 (1) : 133-148, ix.
2. Lee HS. Recent advances in topical anesthesia. J Dent Anesth Pain Med 2016; 16 (4) : 237-44.
3. Johnson SM, Saint John BE, Dine AP. Local anesthetics as antimicrobial agents: A review. Surg Infect (Larchmt) 2008; 9 (2) : 205-13.
4. Kaewjiaranai T, Srisatjaluk RL, Sakdajeyont W, Pairuchvej V, Wongsirichat N. The efficiency of topical anesthetics as antimicrobial agents: A review of use in dentistry. J Dent Anesth Pain Med 2018; 18 (4) : 223-33.
5. Erlich H. Bacteriologic studies and effects of anesthetic solutions on bronchial secretions during bronchoscopy. Am Rev Respir Dis 1961; 84 : 414-21.