A Review on Analytical Methods of Anti-fungal Agents used for the Treatment of Onychomycosis

Abstract

Background: Analytical methods have an essential function in the healthcare sector, particularly when it emanates from the creation and approval of medications. These methods are essential for identifying the active ingredients, ensuring the purity of compounds, and quantifying the existence of any impurities or degradation products. Objective: The objective of the research article is to discuss an analytical method available for the drug Onychomycosis, which is indeed crucial in treating nail infection, to confirm the efficacy and quality of pharmaceutical products. Among various analytical techniques available, UV Spectroscopy, RP-HPLC (high performance-liquid chromatography) and tandem mass spectrometry are normally employed due to their speed and accuracy. Methods: Literature surveys typically reveal the predominance of UV, RP-HPLC and LC-MS/MS methods for estimating azole derivatives due to their established reliability, sensitivity, and applicability to an extensive range of compounds. Researchers often select the most suitable method based on factors such as sample complexity, sensitivity requirements, and available instrumentation. Results: This present art states most of the validation parameters like LOQ, Linearity range, Column and mobile phase used in different dosage forms as well as versatile techniques. Conclusion: This review article provides a comprehensive evaluation of various analytical methods employed in the estimation of antifungal agents, particularly those used to treat Onychomycosis. Onychomycosis, a prevalent infection on nails caused by fungi, demands precise and accurate analytical techniques to ensure the safety and effectiveness of antifungal therapies. In this review, we discussed and compared a range of analytical technologies, highlighting their applications and sensitivity in the context of Onychomycosis drug analysis. This critical assessment aims to guide researchers and practitioners in selecting the most suitable methods for their specific analytical needs.