Affiliation:
1. Department of Pharmacy, University of Malakand, Khyber Pakhtunkhwa, Pakistan
2. Central Department of Chemistry
Tribhuvan University Kirtipur, Kathmandu, Nepal
Abstract
Introduction:
Rosa webbiana (RW) Wall Ex. Royle is used in traditional medicine in
Pakistan for the treatment of several diseases including jaundice. To date, only neuroprotective potential
of the plant has been evaluated.
Objective:
The current study was designed to isolate bioactive compound(s) and investigate its
possible radical scavenging, anti-inflammatory and hepatoprotective activities.
Methods:
Column chromatography was done to isolate compounds from the chloroform fraction
of RW. The compound was characterized by mass spectrometry, 1H-NMR, and 2D-NMR spectroscopy.
Radical scavenging activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen
peroxide (H2O2) assays, while anti-inflammatory potential was evaluated via xylene-induced
ear edema and carrageenan-induced paw edema models. For hepatoprotection, CCl4-induced
model in mice was used.
Results:
A triterpene compound (3α, 21β-dihydroxy-olean-12-ene) was isolated from RW fruits
(ARW1). The compound exhibited DPPH and H2O2 scavenging activities 61 ± 1.31% and 66 ±
0.48% respectively at 500 μg/ml. ARW1 (at 50 mg/kg) exhibited 62.9 ± 0.15% inhibition of
xylene-induced ear edema and 66.6 ± 0.17% carrageenan-induced paw edema in mice. In CCl4-induced
hepatotoxic mice, ARW1 significantly countered elevation in alanine transaminase (ALT),
alkaline phosphatase (ALP), total bilirubin (T.B), and reduction in total protein (T.P) levels. Liver
histomorphological study supported the serum biochemical profile for hepatoprotection. Moreover,
ARW1 significantly attenuated the toxic changes in body and liver weight induced by CCl4.
Conclusion:
The compound ARW1 exhibited anti-radical, anti-inflammatory and hepatoprotective
effects. The anti-inflammatory and hepatoprotective activities may be attributed to anti-oxidant
potential of the compound.
Funder
Higher Education Commission (HEC), Islamabad Pakistan, under NRPU project
Publisher
Bentham Science Publishers Ltd.
Subject
Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Medicine
Reference63 articles.
1. Sini K.; Haribabu Y.; Sajith M.; Sreekumar S. J. J. o. C.; Research P.; in-vitro Cytotoxic activity of Orthosiphon thymi fl orus(Roth.) sleensen leaf extract against dalton lymphoma ascites cell line. J Chem Pharm Res 2012,4(1),917-921
2. Aggarwal B. B.; Kumar A.; Bharti A. C. J. A. r.; Anticancer potential of curcumin: preclinical and clinical studies. Anticancer Res 2003,23(1/A),363-398
3. Cragg G. M.; Newman D. J. J. J. o. e.; Plants as a source of anti-cancer agents. J Ethnopharmacol 2005,100((1-2)),72-79
4. Abdullah M.A.K.; Ahmad Waqar; Ahmad Manzoor; Nisar Mohammad; Hepatoprotective effect of the solvent extracts of Viola canescens Wall. ex. Roxb. against CCl4 induced toxicity through antioxidant and membrane stabilizing activity. BMC Complement Altern Med 2017,17(10)
5. Abdullah M. A. K.; Ahmad W.; Adhikari A.; Ibrar M.; ur Rehman M.; Khan M.; Kamil A.; Exploration of hepatoprotective potential and phytochemicals of ziziphus oxyphylla edgew. Pak Vet J 2020,40(4),431-436