Affiliation:
1. Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology, SASTRA
Deemed University, Thanjavur, 613401, India
Abstract
Background:
The present study aims to develop the use of glutathione copper complex for the
detection of sarcosine, a marker for prostate cancer. The glutathione-copper complex was successfully
synthesized at room temperature and characterized using FTIR, UV, and Scanning Electron Micrograph.
Methods:
The structure of the glutathione copper complex was found to be a 1:2 Metal: Ligand ratio. The
cyclic voltammogram for the glutathione-copper complex modified electrode showed an oxidation peak
around -0.037 V, which indicates the irreversible oxidation of copper ions. The addition of sarcosine decreased
the oxidation potential of the glutathione copper complex indicating the ability of the working
electrode, for the detection of sarcosine in the sample using differential pulse voltammetry.
Results:
The results indicated that the glutathione-copper complex modified electrode revealed good sensitivity,
selectivity, and linearity against the detection of sarcosine in the range from 0.1 μM to 2.5 μM.
The LOD and LOQ were calculated using a linear prediction model, the data obtained from differential
pulse voltammetry technique for known and simulated urine samples was 0.72 μM, 5.13 μM, and 1.45
μM, 39.94 μM, respectively. The sensitivity for the developed working electrode obtained for the known
and simulated sample was 0.0567 μA/μM and 0.02913 μA/μM, respectively.
Conclusion:
Thus, we concluded that the glutathione copper complex decorated glassy carbon electrode
is a good candidate for the detection of sarcosine with good selectivity and sensitivity for real-time monitoring.
Publisher
Bentham Science Publishers Ltd.