Affiliation:
1. Applied Chemistry Department, Faculty of Science, University of Johannesburg, Doornfontein 2028, South Africa
Abstract
Background:
Parabens are synthetic esters used extensively as preservatives and/or
bactericides in personal care personal products.
Objective:
Development and validation of a novel robust chemometric assisted analytical technique
with superior analytical performances for the determination of ethylparaben, methylparaben and
propylparaben, using simulated wastewater matrix.
Methods:
An automated Solid Phase Extraction (SPE) method coupled with liquid chromatographymass
spectrometry was applied in this study. A gradient elution programme comprising of 0.1%
formic acid in deionised water (A) and 0.1% formic acid in Methanol (B) was employed on a 100 x
2.1 mm, 3.0 μm a particle size biphenyl column. Two-level (2k) full factorial design coupled with
response surface methodology was used for optimisation and investigation of SPE experimental
variables that had the most significant outcome of the analytical response.
Results:
According to the analysis of variance (ANOVA), sample pH and eluent volume were
statistically the most significant parameters. The method developed was validated for accuracy,
precision, Limits of Detection (LOD) and Limit of Quantification (LOQ) and linearity. The LOD and
LOQ established under those optimised conditions varied between 0.04-0.12 μgL−1 and 0.14-0.40 μgL−1
respectively. The use of matrix-matched external calibration provided extraction recoveries between
78-128% with relative standard deviations at 2-11% for two spike levels (10 and 100 μgL-1) in three
different water matrices (simulated wastewater, influent and effluent water).
Conclusion:
The newly developed method was applied successfully to the analyses of parabens in
wastewater samples at different sampling points of a wastewater treatment plant, revealing
concentrations of up to 3 μgL−1.
Funder
Water Research Commission
Publisher
Bentham Science Publishers Ltd.
Cited by
4 articles.
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