Applications of CRISPR in Parasitology-Reference-Cited by-同舟云学术

Applications of CRISPR in Parasitology

Published:2024-07-12 Issue: Volume:25 Page:
ISSN:1389-2010
Container-title:Current Pharmaceutical Biotechnology
language:en
Short-container-title:CPB

Author:

Tee Phoebe Yon Ern¹,Chu Sylvester Yee Chun¹,Kok Chloe Chien Yee¹,Foo Mun¹,Tan Clarence Zhen Jin¹,Foo Jhi Biau²³^ORCID,Mohd Hatta Fazleen Haslinda⁴^ORCID,Tan Li Kar Stella²³^ORCID,Hamzah Sharina²³^ORCID

Affiliation:

1. School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University, Subang Jaya, Selangor, 47500, Malaysia

2. School of Pharmacy, Faculty of Health and Medical Sciences, Taylor’s University, Subang Jaya, Selangor, 47500, Malaysia

3. Digital Health & Medical Advancement Impact Lab, Taylor’s University, 47500, Subang Jaya, Selangor, Malaysia

4. Faculty of Pharmacy, Universiti Teknologi MARA UiTM, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia

Abstract

Abstract: Clustered Regions of Interspersed Palindromic Repeat (CRISPR)-based techniques have been utilized in various research areas, including agriculture, biotechnology, and medicine. With the use of a short sequence guide RNA and CRISPR-associated (Cas) protein, this technique allows for robust, site-specific manipulation of the genome, aiding researchers in making important biomedical discoveries and scientific advancements. In this review, we explored the applications of CRISPR/Cas systems in the field of parasitology for the identification and validation of novel functional genes, diagnosis of parasitic infections, reduction of parasite virulence, and the disruption of disease transmission. We also discussed how CRISPR can be used for the development of therapeutics, vaccines, and drug discovery. Furthermore, the challenges and future perspectives of this technology are also highlighted.

Publisher

Bentham Science Publishers Ltd.