Use of Mesenchymal Stem Cells in Experimental Ovarian Damage-Reference-Cited by-同舟云学术

Use of Mesenchymal Stem Cells in Experimental Ovarian Damage

Published:2024-07 Issue:5 Volume:19 Page:725-734
ISSN:1574-888X
Container-title:Current Stem Cell Research & Therapy
language:en
Short-container-title:CSCR

Author:

Fouad Hanan¹²,Albahlol Ibrahim A³⁴,Wahab Hazim A.⁵,Nadwa Eman H⁶⁷,Galal Heba M.⁸⁹,Abouelkheir Mohamed¹⁰¹¹,Taha Ahmed E.¹²¹³,Kamel Abdelkarim G.¹⁴,Abdelmawlla Hassan A.¹⁵¹⁶

Affiliation:

1. Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt

2. Basic Medical Sciences, Faculty of Medicine, Galala University, Galala City, ATAKA, Suez Governorate, Egypt

3. Department of Obstetrics and Gynecology, College of Medicine, Jouf University, Sakaka, Saudi Arabia.

4. Department of Obstetrics and Gynecology, College of Medicine, Mansoura University, Mansoura, Egypt.

5. Department of Histology, Faculty of Medicine, Menofiya University, Shebin Elkom, Egypt

6. Department of Pharmacology and Therapeutics, College of Medicine, Jouf University, Sakaka, Saudi Arabia.

7. Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Giza, Egypt.

8. Department of Medical Physiology, College of Medicine, Jouf University, Sakaka, Saudi Arabia.

9. Department of Medical Physiology, Faculty of Medicine, Assiut University, Assiut, Egypt

10. Department of Pharmacology and Therapeutics, College of Medicine, Jouf University, Sakaka, Saudi Arabia

11. Department of Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

12. Microbiology and immunology unit, Department of Pathology, College of Medicine, Jouf University, Sakaka, Saudi Arabia

13. Department of Medical Microbiology and Immunology, College of Medicine, Mansoura University, Mansoura, Egypt

14. Basic Medical Sciences, Faculty of Medicine, Galala University, Galala City, ATAKA, Suez Governorate, Egypt

15. Department of Anatomy, College of Medicine, Jouf University, Sakaka, Saudi Arabia

16. Department of Anatomy and Embryology, College of Medicine, Beni-Suef University, Beni Suef, Egypt

Abstract

Background: Bisphenol-A (BPA) has a well-proven deleterious effect on the hypothalamicpituitary- gonadal axis. Objectives: The current study investigated the therapeutic potentials of mesenchymal stem cells (MSCs) in a murine model of BPA-induced ovarian damage. Methods: Fifty adult female rats were divided into: Group 1; control group, Group IIa, IIb: rats were given oral gavage of BPA (25 and 50 mg/Kg body weight respectively) on a daily basis for 15 days, and Group IIIa, IIIb; rats were intravenously treated with of MSCs (106 cells) after receiving the last dose of BPA as in group II. Plasma and ovarian tissue levels of Malondialdehyde (MDA) and gonadal axis hormones were assessed. Apoptosis was evaluated by TUNNEL assay and by apoptosis markers (FAS, FASL, Caspase 3, SLTM). A histological examination of ovarian tissue was also conducted. Results: BPA resulted in a significant elevation in plasma levels of LH, FSH, and ovarian tissue levels of MDA and a significant decrease in estradiol and progesterone. All genetic and protein markers of apoptosis were elevated in BPA treated group with decreased oestrogen receptor expression in the ovarian tissue. Increased apoptotic cells were confirmed by TUNEL assay. A high dose of BPA was able to increase the number of atretic follicles in the ovarian tissue whereas the numbers of primordial, primary, secondary and Graafian follicles were decreased. All the laboratory and histological abnormalities were ameliorated by treatment with MSCs. Conclusion: The antioxidant and anti-apoptotic effects of MSCs could possibly explain the ability of this therapeutic modality to ameliorate BPA-induced-ovarian damage.

Publisher

Bentham Science Publishers Ltd.

Subject

General Medicine,Medicine (miscellaneous)