Affiliation:
1. Division of Biomedical Science, School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih,
Selangor Darul Ehsan, Malaysia
2. Department of Pharmacy, International Medical University, Kuala Lumpur,
Malaysia
Abstract
Background:
Agarwood tea derived from Aquilaria malaccensis Lamk is becoming an
increasingly popular herbal drink that is said to have multiple health benefits. Co-administration of
this tea and clinical used drugs is possible, but it increases the risk of drug-herb interactions.
Objectives:
This in vitro study investigated the inhibitory effects of agarwood tea aqueous extract
on the eight major human drug-metabolising cytochrome P450 (CYP) enzyme activities.
Methods:
High-throughput fluorescence-based Vivid® CYP450 screening kits were employed to
obtain the enzyme activities before and after incubation with agarwood tea aqueous extract.
Results:
Agarwood aqueous extract potently inhibited CYP2C9, CYP2D6, and CYP3A4 activities
with Ki values of 5.1, 34.5, and 20.3μg/ml, respectively. The most likely inhibition mode responsible
for these inhibitions was non-competitive inhibition. On the other hand, at 1000μg/ml, agarwood
tea aqueous extract negligibly inhibited CYP1A2, CYP2B6, CYP2C19, CYP2E1, and
CYP3A5 activities.
Conclusion:
These findings can be used to design additional in vitro investigations using clinical
relevant drug substrates for CYP2C9, CYP2D6, and CYP3A4. Subsequently, future studies can be
conducted to determine potential interactions between agarwood tea aqueous extract and CYP
using in vivo models.
Funder
University of Nottingham Malaysia
Publisher
Bentham Science Publishers Ltd.
Cited by
2 articles.
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