Affiliation:
1. Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
Abstract
Background:
Osteosarcoma is a highly invasive and early metastatic tumor. At present, the toxic
and side effects of chemotherapy affect the quality of life of cancer patients to varying degrees. Genipin is an
extract of the natural medicine gardenia with various pharmacological activities.
Objective:
The purpose of this study was to investigate the effect of Genipin on osteosarcoma and its potential
mechanism of action.
Methods:
Crystal violet staining, MTT assay and colony formation assay were used to detect the effect of
genipin on the proliferation of osteosarcoma. The effects of vitexin on migration and invasion of osteosarcoma
were detected by scratch healing assay and transwell assay. Hoechst staining and flow cytometry were used to
detect the effect of genipin on apoptosis of osteosarcoma cells. The expression of related proteins was detected
by Western blot. An orthotopic tumorigenic animal model was used to verify the effect of genipin on osteosarcoma
in vivo.
Results:
The results of crystal violet staining, MTT method and colony formation method proved that genipin
significantly inhibited the proliferation of osteosarcoma cells. The results of the scratch healing assay and transwell
assay showed that gen significantly inhibited the migration and invasion of osteosarcoma cells. The results
of Hoechst staining and flow cytometry showed that genipin significantly promoted the apoptosis of osteosarcoma
cells. The results of animal experiments show that genipin has the same anti-tumor effect in vivo.
Genipin may inhibit the growth of osteosarcoma through PI3K/AKT signaling.
Conclusion:
Genipin can inhibit the growth of human osteosarcoma cells, and its mechanism may be related to
the regulation of PI3K/AKT signaling pathway.
Funder
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmacology
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献