Meta-analysis of the Mesenchymal Stem Cells Immortalization Protocols: A Guideline for Regenerative Medicine

Author:

Elias Ferreira Stricker Priscila1ORCID,Barth de Oliveira Nathalia1ORCID,Felipe Mogharbel Bassam1ORCID,Lührs Larissa1ORCID,Irioda Ana Carolina1ORCID,Abdelwahid Eltyeb2ORCID,Regina Cavalli Luciane1ORCID,Zotarelli-Filho Idiberto José343ORCID,Athayde Teixeira de Carvalho Katherine1ORCID

Affiliation:

1. Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties, Curitiba, Brazil

2. Feinberg School of Medicine, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL, USA

3. College of Palliative Medicine of Sri Lanka, Colombo, Sri Lanka

4. ABRAN - Associação Brasileira de Nutrologia/Brazilian Association of Nutrology, Catanduva, Sao Paulo, Brazil

Abstract

Background:: This systematic review describes the most common methodologies for immortalizing human and animal mesenchymal stem cells (MSCs). This study follows the rules of PRISMA and is registered in the Institutional Review Board of PROSPERO International of systematic reviews, numbered protocol code: CRD42020202465. Method:: The data search systematization was based on the words “mesenchymal stem cell” AND “immortalization.” The search period for publications was between 2000 and 2022, and the databases used were SCOPUS, PUBMED, and SCIENCE DIRECT. The search strategies identified 384 articles: 229 in the SCOPUS database, 84 in PUBMED, and 71 in SCIENCE DIRECT. After screening by titles and abstracts, 285 articles remained. This review included thirty-nine articles according to the inclusion and exclusion criteria. Result:: In 28 articles, MSCs were immortalized from humans and 11 animals. The most used immortalization methodology was viral transfection. The most common immortalized cell type was the MSC from bone marrow, and the most used gene for immortalizing human and animal MSCs was hTERT (39.3%) and SV40T (54.5%), respectively. Conclusion:: Also, it was observed that although less than half of the studies performed tumorigenicity assays to validate the immortalized MSCs, other assays, such as qRT-PCR, colony formation in soft agar, karyotype, FISH, and cell proliferation, were performed in most studies on distinct MSC cell passages.

Publisher

Bentham Science Publishers Ltd.

Subject

General Medicine,Medicine (miscellaneous)

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