Isolation, characterization, and antiproliferative activity of polymethoxy flavones from Mentha dumetorum

Author:

Akşit Hüseyin1ORCID,Ozan Erdem2ORCID,Erenler Ramazan3

Affiliation:

1. Faculty of Pharmacy, Erzincan University, Erzincan Binali Yıldırım Üniv. Yalnızbağ yerleşkesi Eczacılık Fakültesi Erzincan, Turkey

2. Department of Chemistry, Tokat Gaziosmanpasa University, tokat, Turkey

3. Department of Chemistry, Tokat Gaziosmanpasa University, Turkey

Abstract

Background: Natural products have been used commonly in the pharmaceutical industry as well as traditional medicine due to their bioactive contents. Objective: The objective of this study is to isolate the polymethoxy flavones (PMFs) and evaluate the antiproliferative activity. Method: The PMFs were extracted from Mentha dumetorum with boiling hot water and then partitioned with hexane and the extract was subjected to chromatographic techniques such as Sephadex LH-20, silica gel, and preparative TLC, and HPLC to isolate the compounds. The structures of isolated compounds were elucidated by spectroscopic techniques such as 1H-NMR, 13C-NMR, LC-TOF-MS, and UV-Vis. The anti-cancer effects of isolated compounds were evaluated using a real-time cell analyzer–single plate (RTCA-SP) instrument against HeLa and HT29 cell lines. Results: The isolated PMFs were identified as xanthomicrol (1), 5-hydroxy-6,7,8,4'-tetrametoxy flavanone (2), 5-desmetil sinensetin (3), 5-demetil nobiletin (4), gardenin B (5), 5-hydroxy-6,7,8,3',4'-penta methoxy flavanone (6) and 5-hydroxy-6,7,4'-trimethoxy flavone (7). Compounds 2 and 5 were found to be the most active against both cell lines. Conclusion: The isolated compounds as well as the plant extract of Mentha dumetorum could be promising agents for the drug development process, especially drugs for cancer treatment. Moreover, isolation methods were developed for the corresponding compounds.

Publisher

Bentham Science Publishers Ltd.

Subject

Complementary and alternative medicine,Drug Discovery

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