Affiliation:
1. Department of Chemistry, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Malaysia
2. Department of Chemistry, Kaduna State University (KASU), P.M.B 2339, Tafawa Balewa way, Kaduna, Nigeria
Abstract
Background:
Deinbollia pinnata belonging to Sapindaceae family are known as
medicinal plants for the treatment of human diseases. Because of human over-exploitation, the
choice of phytochemicals re-search and screening was made to add to the available compounds and
ethno-medicinal records.
Objective:
To investigate phytochemicals and their antioxidant activities.
Methods:
Antioxidant assay (DPPH, ABTS, FRAP) activities; phytochemical contents (Folin
Ciocalteaus’s (FC) and aluminum chloride) methods; and phytochemical analysis were employed.
Successful characterization of isolates using spectroscopic methods (1H NMR, 13C NMR, FTIR and
GC-MS analysis).
Results:
Alkaloid, simple sugar, phenolics, tannins and steroids were present in all the parts.
Saponin, terpenes and flavonoids were found in both leaves and root bark, while root bark and
heartwood possessed quinones. The methanol extracts demonstrated total phenolic content at 75. 9 ±
2.05 GAE (mg GAE/g dw) and total flavonoid content at 88.41 ± 0.56 QUE (mg QUE/g dw). The
plant extracts showed good DPPH scavenging activity with percentage inhibition more than 70% at
125 ppm. The methanol extracts showed excellent antioxidant activity towards ABTS assay with the
IC50 value of 13.68 μg/mL. The extracts also exhibited the most potent ferric ion reducer at 3.45 ±
1.30 mM FRAP equivalent. Methyl gallate (2) displayed the highest antioxidant radical scavenging
activity towards DPPH assay with the IC50 value of 0.19 μg/mL and pyrogallol (4) showed the highest
ABTS activity with the SC50 value of 7.44 μg/mL. While, 3,4-dihydroxybenzoic acid (1) also
showed the potential as ferric ion reducer ranging from 0.67± 0.01 to 3.63±0.12 mM FRAP
equivalent respectively.
Conclusion:
ased on the antioxidant activity, methanolic extracts and its isolated constituents can
be used as antioxidant sources for the management of oxidative causative diseases. Being the first
report of these phytochemicals from genius Deinbollia and specie pinnata.
Funder
Ministry of Higher Education (MOHE) Malaysia
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery