Affiliation:
1. Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, Université Ferhat ABBAS Sétif-1,
19000 Sétif, Algeria
2. Research Center of Biotechnology (CRBt), Ali Mendjli, 25000 Constantine, Algeria
3. Department of Microbiology and Biochemistry, Faculty of Science, Mohamed Boudiaf University, M’sila 28000,
Algeria
Abstract
Aim:
This study investigates the potential effect of pure curcumin on the inhibition of different
enzymes involved in several diseases.
Background:
Several chronic diseases such as Alzheimer’s, diabetes, and Parkinson’s are related to oxidative
stress and enzyme activity. Today, various plant origin products are beneficial against several
chronic diseases with secondary metabolites such as phenolic compounds. Curcumin, a polyphenol yellow-
orange pigment in turmeric spices, has a wide range of biological activities with quite a safety.
Objective:
This study was aimed to investigate the antioxidant and inhibitory potential against key enzymes involved in human pathology, namely Alzheimer's disease (Acetylcholinesterase (AChE), and Butyrylcholinesterase (BChE)), diabetes (α-glucosidase) and hyperpigmentation and Parkinson’s diseases (Tyrosinase) of curcumin.
Methods:
1,1-diphenyl-2-picrylhydrazyl free radical (DPPH●) and hydrogen peroxide radicals (H2O2)
assays were used to evaluate the antioxidant capacity of curcumin, and enzyme inhibitory activity was
evaluated using in vitro standard procedures.
Results:
Curcumin exhibited an excellent antioxidant effect with an IC50 value significantly less than
Vit C reference. In enzyme inhibitory activity, curcumin demonstrated excellent inhibitory activity
against AChE, BChE and α-glucosidase. The finding showed that curcumin was significantly less than
the reference galantamine against AChE but more than the references galantamine and acarbose against
BChE and α-glucosidase, respectively. Whereas for anti-tyrosinase activity, curcumin displayed weak
inhibitory activity compared with the standard inhibitor, Kojic acid.
Conclusion:
These results indicated that curcumin showed promising antioxidant, anti-Alzheimer, and
anti-diabetic properties and might be used as potential natural drugs against these diseases.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,Biochemistry
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