Affiliation:
1. Nutrition and Food Technology Group, Food Engineering Department, Faculty of Pharmaceuticals and Food Sciences, University of Antioquia, Medellín, Colombia
Abstract
Background:
The animal blood that is produced in a slaughterhouse is a potential source
of inexpensive proteins used in the food industry around the world. However, 60% of it is surplus,
and it ends with a negative environmental impact.
Introduction:
The enzymatic hydrolysis of proteins represents a good way to produce peptides with
different biological activities.
Methods:
Enzymatic hydrolysis of bovine plasma with subtilisin at an alkaline pH and 61.5°C was
performed using the pH-stat method. Experiments were conducted considering the effects of a high
initial substrate concentration (So) and the enzyme/substrate ratio (E/S) minimizing the processing
time necessary to obtain a specific degree of hydrolysis (DH).
Results:
The best conditions obtained were 42 g/L of So and 0.89 AU/g substrate of E/S until a DH
of 20% in 11,1 ± 1,1 min was achieved to the tested conditions, which result in a fitted empirical
polynomial equation of degree 3.
Conclusion:
A kinetic equation is established to relate the DH and the reaction time to a relative error
of less than 5% in the fit, to obtain a good antioxidant product in an industrially interesting time.
Additionally, the results suggest a good adjustment of the data with a determination coefficient (R2)
of 0.9745 in validation.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,Biochemistry
Cited by
5 articles.
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