Affiliation:
1. Department of Biosciences, Integral University, Lucknow-226026,, India
Abstract
Introduction:
The increased level of ethylene inhibits root elongation and
causes physiological damage, thereby reduces ethylene level imparts a positive support
against various biotic and abiotic stresses viz. phytopathogens, extreme temperatures,
hyper salinity, flooding, drought, metal/organic contaminants and insect
predation. The metabolic pathways showed the involvement of ACC synthase inhibition
for ethylene suppression in plants.
Objective:
The primary objective of this study focused towards the use of In-silico
approach to assess the inhibitory effect of S-adenosyl methionine (SAM) analogue
on ACC synthase activity.
Methods:
The 3D structure of ACC synthase of Pisum sativum was constructed using modeler 9v11
software. The reliability of developed model was evaluated by PROCHECK, ERRAT and ProSA web
servers. Furthermore the molecular interactions between substrate SAM and inhibitors were performed.
Result:
The docking study demonstrated that the binding energy of the substrate SAM is -5.37
Kcal/mol. The SAM analogue (Inhibitors) considered in this study were 3-dzSAHC, SAHC, sinefungin,
SIBA, 7-dz-SIBA, 1-dz-SIBA,3-dz-SIBA and S-n-Butyladenosine. Among these analogues, 7-dz-SIBA
was found to be most effective on ACC synthase as inhibitor due to lowest binding energy (-
5.51Kcal/mol) and strong Ki value (91.74M). The LYS276 amino acid residue of ACC synthase was
observed in the interaction with both substrates SAM and 7-dz-SIBA (S-isobutyl-7-deazaadenosine)
demonstrates as most crucial catalytic residue for molecular interaction.
Conclusion:
This study successfully screened most potent inhibitor for ACC synthase which have
indicated the compounds 7-dz- SIBA as effective inhibitor with lowest binding energy, better hydrogen
bond interaction and strong inhibition constant compared to others compounds studied. Thus 7-dz-SIBA
can be projected to use as a growth enhancer for overall crop improvement. It may help in plant growth
promotion, prevents the plants from various environmental stress and phytopathogenic infections etc.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,Biochemistry
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献