The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium-Reference-Cited by-同舟云学术

The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium

Published:2020-12-21 Issue:8 Volume:21 Page:602-609
ISSN:1389-2029
Container-title:Current Genomics
language:en
Short-container-title:CG

Author:

Gao Caixia¹,Zhang Mingnan¹,Chen Lei¹

Affiliation:

1. Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

Abstract

The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them.

Funder

Shanghai Municipal Commission of health and family planning

Publisher

Bentham Science Publishers Ltd.

Subject

Genetics(clinical),Genetics

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