Establishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples

Author:

Ma Hong1,Liao Dezhong J.234,Zhang Keyin235,Yang Jianglin56,Qin Zhenwei7,Lu Tianzu1,Lou Didong7,Ran Qianchuan7,Huang Hai6,Cheng Shuqiang6,Zellmer Lucas8

Affiliation:

1. Department of Stomatology, School of Stomatology, Guizhou Medical University, 4 Beijing Road, Guiyang 550004, Guizhou Province, P.R. China

2. Department of Pathology, School of Clinical Medicine, Guizhou Medical University, 4 Beijing Road, Guiyang 550004, Guizhou Province, P.R. China

3. Department of Pathology, The Affiliated Hospital of Guizhou Medical University, 4 Beijing Road, Guiyang 550004, Guizhou Province, P.R. China

4. Key Lab of Endemic and Ethnic Diseases of the Ministry of Education of China in Guizhou Medical University, Guiyang 550004, Guizhou Province P. R. China

5. Key Lab of Endemic and Ethnic Diseases of the Ministry of Education of China in Guizhou Medical University, Guiyang 550004, Guizhou Province, P. R. China

6. Center for Clinical Laboratories, Guizhou Medical University Hospital, 4 Beijing Rd, Guiyang 550004, Guizhou Province, P.R. China

7. Forensic Science Section, School of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Dong-Qing-Nan Road, Guiyang 550025, Guizhou Province, P.R. China

8. Department of Medicine, Hennepin County Medical Center, 730 South 8th St., Minneapolis, MN 55415

Abstract

Background:The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods.Methods:We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers.Results:Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, i.e. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified using PCR and ensuing visualization of the PCR amplicons in agarose gels. Most of the tested and untested sex markers possess significant differences in the molecular weight between the X- and Y-derived PCR amplicons and are thus much better than most, if not all, previously-reported genetic sex markers. We also established several simple and essentially cost-free methods for extraction of crude genomic DNA from cultured cells, blood samples, and tissues that could be used as template for PCR amplification.Conclusion:We have established new sex genetic markers and methods for extracting genomic DNA and for sexing human and mouse cells. Our work may also lend some methodological strategies to the identification of new genetic sex markers for other organismal species.

Publisher

Bentham Science Publishers Ltd.

Subject

Genetics (clinical),Genetics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3