Chlorambucil Cytotoxicity Reduction in Rats Through Bone Marrow, An In vivo Study
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Published:2019-02-28
Issue:15
Volume:18
Page:2187-2192
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ISSN:1871-5206
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Container-title:Anti-Cancer Agents in Medicinal Chemistry
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language:en
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Short-container-title:ACAMC
Author:
Nafie Ebtesam H.O.1,
Khater Eman1,
Awwad Mohamed1,
Zowail Mohamed1,
Hegazy Kholud1
Affiliation:
1. Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt
Abstract
Background:
Bone Marrow (BM) has the self-renovation capacity and has been used recently in tumor
medicine. Chlorambucil [CHB] is ordinarily utilized chemotherapy to treat varieties of malignancy patients. This
investigation intended to gauge the effectiveness of BM as an in-vivo antimutagenic against CHB.
Methods:
The experimental design relies upon four classes; each class contains ten adult male albino rats as follows:
control, rats infused orally with CHB for fourteen days, rats intravenously infused with BM through a tail vein one
time, rats infused the mix of CHB and BM.The Anticancer capability of BM was assessed by cytogenetic assay and
mitotic index. The declarations of the apoptosis- related genes were examined by RT-qPCR examination.
Results:
The present experiment demonstrated a curative effect of BM against the cytotoxic impact of CHB. Infusion
of BM after chemotherapy helps to diminish the chromosomal aberration; increment mitotic index and decline the
Bax/Bcl2 proportion compared with [CHB] class gather that prompts expanding the survival rate of influenced cells
with chemotherapy cytotoxicity.
Conclusion:
The present study shows that bone marrow transplantation together after CHB infusion helps to increase
genomic stability by diminishing structural chromosome abnormalities, diminishing the Bax/Bcl2 proportion and
increasing the mitotic index.
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine