Affiliation:
1. Department of Dermatology, Sanglah Hospital, Faculty of Medicine, Udayana University, Denpasar, Indonesia
2. Pediatric Consultant, Critical Care Medicine Udayana University, Sanglah Hospital, Denpasar, Indonesia
3. Pharmacy Department, Faculty of Mathematics and Natural Science, Udayana University, Indonesia
Abstract
Background:
Andrographolide (AP), a bioactive anti-inflammatory compound of Sambiloto,
inhibits NF-κB, TNF-α, and interleukin IL-6. Nowadays, molecular docking simulation between
AP and dexamethasone against NF-κB receptor presented the energy AP higher than dexamethasone.
This becomes a potential treatment for psoriasis.
Objective:
This manuscript reported the effectiveness of AP from Sambiloto in treating psoriasis
compared to topical steroids.
objective:
Thus, this manuscript reported the effectiveness of AP from Sambiloto in treating psoriasis compared to topical steroids.
Methods:
This study conducted TLC analysis of AP content and its metabolite impurities, emulgel
formulation, molecular docking, in-silico skin toxicity study, and in-vivo anti-psoriatic activity.
This was a combination study of an in-silico study and an in-vivo study. This in-silico study
was analyzed through multivariate statistical analysis (PCA) to elucidate the data constellation relationship
of andrographolide derivatives with several target proteins. The intervention was performed
in seven days. The PASI score, molecular parameters (IL-6, IL-17, VEGF, and TNF-a levels),
and histopathological findings were assessed.
Results:
Molecular docking results revealed andrographolide to exhibit a relatively high binding
affinity towards IL-6, NF-kB, and TNF-α which is comparable to the corticosteroids, andrographolide
also shares similar residue interaction profile with each of the respective protein’s native
ligand. In the in-vivo study, we found several parameters statistically significantly different regarding
the intervention, including final PASI score (p = 0.017), redness (p = 0.017), scale (p =
0.040), thickness (p = 0.023), total histopathology of psoriasis score (p = 0.037), keratin layer
score (p = 0.018).
Conclusion:
Emulgel AP 0.1% could lower the anti-inflammatory agent, which is vital to psoriasis
progression.
Publisher
Bentham Science Publishers Ltd.