Affiliation:
1. Amity
Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow-226028, India
2. Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli - 627 012, Tamil Nadu, India
Abstract
Background:
The insulin receptor protein of humans is proposed to have substantial application
in the regulatory pathway of glucose levels in the blood. The cellular function of the insulin hormone
is initiated by its association with the insulin receptor (IR) protein, and this process is common to most
vertebrate species. Thus, phylogenetic analysis of IR protein among various associated species in vertebrates
can elucidate the importance of residues and structure, and the relationship with its function as the
conserved region of residues is associated with evolutionary conservation on insulin receptor proteins. In
the present study, we have used various in silico approaches and conducted the phylogenetic analysis.
Method:
The basic local alignment search tool (BLAST) was employed to screen the 250 vertebrates.
After evolutionary analysis, Pan troglodytes (Chimpanzees) have been observed as the closest relatives of
Homo sapiens concerning insulin receptor proteins.
Results:
Human insulin receptor protein was found to have hydrophobic nature by hydrophobicity profile
analysis, where most of the positions were above the mean hydrophobicity value from n-terminal to cterminal
residues, whereas carboxy-terminal residues were observed as hydrophilic. High entropy was
observed at the short stretch at N-terminal, C-terminal, and a linker region within the alignment, but the
overall positions which were aligned showed low entropic regions.
Conclusion:
The outcomes of this work have revealed some unexplored specific characteristics of the
conserved domains among different taxa of selected vertebrates and have also illustrated the hierarchical
assemblage-based inconsistent variation of the IR proteins, and further conservancy analysis discloses the
significance of each site for protein structure or function.
Funder
DST-INSPIRE Govt. of India
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmaceutical Science,Molecular Medicine