Affiliation:
1. College of Chemistry and Environmental Science, Qujing Normal University, Qujing 655011, China
2. R&D Center of China Tobacco Yunnan Industry Co., Ltd., Kunming 650231, China
3. Department of Pharmacy and Instruments, Qujing First People’s Hospital, Qujing 655000, China
Abstract
Background:
The Bai ethnologic herb Radix Dactylicapnotis, the root and tuber of Dactylicapnos
scandens (Papaveraceae), is used for clearing heat, relieving pain, and achieving hemostasis
and antihypertensive effects.
Objective:
The study aimed to develop a quantitative method for determining the protopine content in
Radix Dactylicapnotis by using proton nuclear magnetic resonance (1H NMR) spectroscopy.
Methods:
The deuterium solvent, internal standard, and NMR parameters were optimized. The quantitative
method was validated by linearity, precision, accuracy, repeatability, and stability, as well as
limit-of-detection (LOD) and limit-of-quantitation (LOQ) assays.
Results:
A mixture solution consisting of 500 μL of DMSO-d6 and 20 μL of D2O enabled satisfactory
separation of the signals to be integrated into the 1H NMR spectrum. Trimethyl benzene-1,3,5-
tricarboxylate (TMBT) was selected as an internal standard. The integration of δ 6.05-6.08 corresponding
to OCH2O was selected to quantify protopine. The developed quantitative method was
found to be precise and accurate and to exhibit excellent linearity and range. The protopine content in
Radix Dactylicapnotis could be quantified accurately using the featured signal.
Conclusion:
This is the first study to report quantitative 1H NMR determination of protopine in Radix
Dactylicapnotis. The study results indicate that quantitative 1H NMR represents a feasible alternative
to HPLC-based methods for the quantitation of protopine in Radix Dactylicapnotis, and is suitable for
the quality control of Radix Dactylicapnotis.
Funder
Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities
Program for Innovative Research Team at Qujing Normal University, Science Foundation from Yunnan Provincial Department of Education
Undergraduates Innovative Experiment Project from MOE, China
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics