Affiliation:
1. Department of Biology, Faculty of Science, Lorestan University, Khorramabad, Iran
2. Razi Herbal Medicines
Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3. Department of Medical Parasitology
and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract
Background:
One of the most important principles in disease control is the health of livestock
and poultry feed. Given the natural growth of Th. eriocalyx in Lorestan province, its essential oil
can be added to the livestock and poultry feed and prevent the growth of the dominant filamentous
fungi.
Objective:
Therefore, this study aimed to identify the dominant moldy fungal agents of livestock and
poultry feed, examine phytochemical compounds and analyze antifungal effects, anti-oxidant properties,
as well as cytotoxicity against human white blood cells in Th. eriocalyx.
Methods:
Sixty samples were collected in 2016. The PCR test was used to amplify ITS1 and ASP1
regions. The analysis of essential oil was conducted by gas chromatography and gas chromatographymass
spectrometry devices. MIC and MFC were performed using the broth micro-dilution method.
For the analysis of DDPH activity, DDPH was used. Cytotoxicity effect on healthy human lymphocytes
was carried out by the MTT method.
Results:
In this study, A. niger, F. verticilloides and F. circinatum, P. oxalicum, and P. chrysogenum
were the most resistant species, and A. oryzae and A. fumigatus, F. prolifratum and F. eqiseti, P.
janthnellum were the most susceptible ones. IC50 value of T. daenensis Celak was 41.33 μg/ml, and
100 μl/ml of the essential oil caused slight cell lysis.
Conclusion:
Considering our results, compared with drugs and chemical additives, essential oils can
be added to livestock and poultry feed to prevent the growth of filamentous fungi in the livestock and
poultry feed.
Funder
Lorestan University of Medical Sciences, Khorramabad, Iran
Publisher
Bentham Science Publishers Ltd.
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