Affiliation:
1. Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh- 201301, India
2. Department of Pharmaceutical Sciences, University of Kashmir-190006, India
3. CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu- 180001, India
Abstract
Background:
Pentacyclic triterpenoids are a biologically active class of
phytoconstituents with diverse pharmacological activities, including anti-inflammatory action.
:
In the current study, we isolated 3-Acetylmyricadiol, a pentacyclic triterpenoid, from
the ethyl acetate bark extract of Myrica esculenta and evaluated it for anti-inflammatory potential.
Methods:
The ethyl acetate bark extract of the M. esculenta was subjected to column
chromatography to isolate 3-Acetylmyricadiol. MTT assay was performed to check cell viability.
The production of proinflammatory mediators like nitric oxide, IL-6, TNF-α were observed after
the administration of 5, 10, 20 μM of 3-Acetylmyricadiol in LPS-activated raw 246.7 macrophages
by the reported methods.
Results:
MTT assay indicated more than 90% cell viability up to 20 μM of 3-Acetylmyricadiol.
The administration of 3-Acetylmyricadiol inhibited the production of nitric oxide, IL-6, TNF-α in
a dose-dependent manner significantly in comparison to LPS treated cells. The maximum effect
was observed at 20 μM of 3-Acetylmyricadiol which resulted in 52.37, 63.10, and 55.37 %
inhibition of nitric oxide, IL-6, and TNF-α, respectively.
Conclusion:
Our study demonstrated the anti-inflammatory action of 3-Acetylmyricadiol and can serve
as a potential candidate in the development of the clinically efficient anti-inflammatory molecule.
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine
Cited by
17 articles.
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