Identification of Differentially Expressed Proteins in Rats with Early Subacute
Spinal Cord Injury using an iTRAQ-based Quantitative Analysis
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Published:2023-09
Issue:11
Volume:26
Page:1960-1973
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ISSN:1386-2073
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Container-title:Combinatorial Chemistry & High Throughput Screening
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language:en
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Short-container-title:CCHTS
Author:
Lou Yongfu12, Lv Yigang12, Li Zhen12, Kang Yi12, Hou Mengfan12, Fu Zheng3, Lu Lu12, Liu Lu4, Cai Zhiwei5, Qi Zhangyang12, Jian Huan12, Shen Wenyuan12, Li Xueying6, Zhou Hengxing127, Feng Shiqing127
Affiliation:
1. Department of Orthopaedics, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin
300052, China 2. Tianjin Key Laboratory of Spine and Spinal Cord Injury, Department of Orthopedics, International
Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Medical University General Hospital, Tianjin
300052, China 3. Key Laboratory of Immuno Microenvironment and Disease of the Educational Ministry of China, Department
of Immunology, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, China 4. Department of Traumatic Orthopedics, Honghui Hospital, Xi'an Jiaotong University, 555 West Youyi Road, Xi'an,
710061, Shaanxi, China 5. Department of Burn and Plastic Surgery, Burns Institute, Burn & Plastic Hospital of PLA
General Hospital, Fourth Medical Center of PLA General Hospital, Beijing, 100048, China 6. Shandong University Centre
for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan,
Shandong 250012, P.R. China 7. Department of Orthopaedics, Shandong University Centre for Orthopaedics, Qilu Hospital,
Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China
Abstract
Background:
Injuries to the central nervous system (CNS), such as spinal cord injury
(SCI), may devastate families and society. Subacute SCI may majorly impact secondary damage
during the transitional period between the acute and subacute phases. A range of CNS illnesses has
been linked to changes in the level of protein expression. However, the importance of proteins during
the early subacute stage of SCI remains unknown. The role of proteins in the early subacute
phase of SCI has not been established yet.
Methods:
SCI-induced damage in rats was studied using isobaric tagging for relative and absolute
protein quantification (iTRAQ) to identify proteins that differed in expression 3 days after the injury,
as well as proteins that did not alter in expression. Differentially expressed proteins (DEPs)
were analyzed employing Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and
Genomes (KEGG) analysis to discover the biological processes, cell components, and molecular
functions of the proteins. We also performed Gene Set Enrichment Analysis (GSEA) software BP
pathway and KEGG analysis on all proteins to further identify their functions. In addition, the first
15 key nodes of a protein-protein interaction (PPI) system were found.
Results:
During the early subacute stage of SCI, we identified 176 DEPs in total between the control and damage groups, with 114 (64.77%) being up-regulated and 62 (35.23%) being down-regulated. As a result of this study, we discovered the most important cellular components and molecular activities, as well as biological processes and pathways, in the early subacute phase of SCI. The top 15 high-degree core nodes were Alb, Plg, F2, Serpina1, Fgg, Apoa1, Vim, Hpx, Apoe, Agt, Ambp, Pcna, Gc, F12， and Gfap.
Conclusion:
Our study could provide new views on regulating the pathogenesis of proteins in the
early subacute phase after SCI, which provides a theoretical basis for exploring more effective
therapeutic targets for SCI in the future.
Funder
National Natural Science Foundation of China Taishan Scholars Program of Shandong Province-Young Taishan Scholars National Key Research and Development Project of Stem Cell and Transformation Research Science & Technology Development Fund of Tianjin Education Commission for Higher Education
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine
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