Affiliation:
1. Department of Respiratory and Critical Care Medicine, Shanghai Changhai Hospital, The First Affiliated Hospital of
Second Military Medical University, Shanghai 200433, People’s Republic of China
Abstract
Background:
Non-small cell lung cancer (NSCLC) has been the subject of intense
scholarly debate. We aimed to identify the potential biomarkers via bioinformatics analysis.
Methods:
Three datasets were downloaded from gene expression omnibus database (GEO). R
software was applied to screen differentially expressed genes (DEGs)and analyze immune cell infiltrates.
Gene set enrichment analysis (GSEA) showed significant function and pathway in two
groups. The diagnostic markers were further investigated by multiple machine learning algorithms
(least absolute shrinkage and selection operator (LASSO) and support vector machine-recursive
feature elimination (SVM-RFE)). Various online analytic platforms were utilized to explore the
expression and prognostic value of differential genes. Furthermore, western blotting was performed
to test the effects of genes on cell proliferation in vitro.
Results:
We identified 181 DEGs shared by two datasets and selected nine diagnostic markers.
Those genes were also significantly overexpressed in the third dataset. Topoisomerase II alpha
(TOP2A) is overexpressed in lung cancer and associated with a poor prognosis, which was confirmed
using immunohistochemistry (IHC) and western blotting. Additionally, TOP2A showed a negative
correlation with immune cells, such as CD8+ T cells, eosinophils and natural killer (NK) cell.
Conclusion:
Collectively, for the first time, we applied multiple machine learning algorithms,
online databases and experiments in vitro to show that TOP2A is a potential biomarker for lung
adenocarcinoma and could facilitate the development of new treatment strategies.
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献