Potential Bioactive Components of Manisrejo Leaf Extract Downregulate MDA and Upregulate Monocytes in Rabbits

Abstract

Introduction:Antioxidants derived from herbal plants can reduce malondialdehyde (MDA) and increase monocytes in experimental animals. The administration of manisrejo leaf extract is expected to reduce MDA and increase monocytes. Manisrejo leaves, which are still closely related to berries (Vaccinium sp), have been widely studied as natural antioxidants. This study aimed to elucidate the potential bioactive of manisrejo leaf extract against MDA and monocytes in rabbits.Methods:This study applied the posttest-only control group design. The object of the study was 20 male broiler rabbits aged 2 months treated with manisrejo leaf extract. During the 28 days, the objects were divided into 5 groups; P0 was given normal feed, P1 normal feed plus manisrejo leaf extract as much as 2.5 g/kg BW, P2 normal feed plus manisrejo leaf extract as much as 5 g/ kg BW, P3 normal feed plus manisrejo leaf extract as much as 10 g/kg BW, and P4 normal feed plus manisrejo extract as much as 20 g/kg BW.Results:The data were processed using SPSS using OneWay ANOVA and Kruskal-Wallis tests. The results showed albumin levels (p = 0.899), AST (Aspartate transaminase) levels (p = 0.198), ALT (Alanine Aminotransferase) levels (p = 0.216), cholesterol levels (p = 0.995), monocyte levels (p = 0.014), lymphocyte levels (p = 0.903), leukocyte levels (p = 0.934), neutrophil segment levels (p = 0.210), MDA levels (p = 0.001), and CD44 levels (p = 0.894).Conclusion:Manisrejo leaf extract may contain some potential bioactives that affect downregulating MDA levels and upregulating monocytes in rabbits.