Affiliation:
1. Faculty of Medicine, University Monastir, LR12ES05, Lab-NAFS ‘Nutrition - Functional Food & Vascular Health’, Monastir, Tunisia
2. University Bourgogne Franche-Comte, Team, Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism, EA 7270 / Inserm, Dijon, France
Abstract
Background:
Oxidative stress is the main feature of several diseases including Alzheimer’s
disease (AD). The involvement of oxysterols derivates has been recently reported.
Objective:
The aim of this study was to evaluate the implication of oxidative stress in cholesterol
impairment in AD patients.
Methods:
A case-control study was conducted on 56 AD patients and 97 controls. Levels of oxidative
biomarkers, including lipid peroxidation products and antioxidant enzyme activities were measured with
spectrophotometric methods on red blood cells (RBCs) and plasma. Cholesterol precursors and oxysterols
(7-Ketocholeterol (7KC), 7α-hydroxycholesterol (7α-OHC), 7β-hydroxycholesterol (7β-OHC), 24Shydroxycholesterol
(24S-OH), 25-hyroxycholesterol (25-OHC), and 27-hydroxycholesterol (27-OHC),
in plasma were quantified by gas chromatography coupled with mass spectrometry.
Results:
In RBCs and plasma of AD patients, a significant decrease of glutathione peroxidase (GPx)
activity was detected associated with raised levels of malondialdehyde (MDA). A decreased level of
lanosterol and an accumulation of 7β-OHC, 24S-OHC, 27-OHC, and 25-OHC that were higher in
plasma of AD patients, compared to controls, were also observed in AD patients. Mini-Mental State Examination
(MMSE) score was correlated with MDA and conjugated dienes (CD) levels in plasma. Besides,
the MDA level in RBCs was correlated with 7β-OHC. Binary logistic regression revealed an association
between GPx activity and AD (OR=0.895, 95%CI: 0.848-0.945. P<0.001).
Conclusion:
Our data consolidate the relationship between the rupture of redox homeostasis and lipid
and cholesterol oxidation in AD.
Publisher
Bentham Science Publishers Ltd.
Subject
Clinical Neurology,Neurology
Reference83 articles.
1. Sies H.; Jones D.; Oxidative stress Encyclopedia of stress Fink G Ed 2nd ed Elsevier: Amsterdam 2007,3: pp.,45-8
2. Andreyev A.Y.; Kushnareva Y.E.; Starkov A.A.; Mitochondrial metabolism of reactive oxygen species. Biochemistry (Mosc) 2005,70(2),200-214
3. Karelson E.; Bogdanovic N.; Garlind A.; The cerebrocortical areas in normal brain aging and in Alzheimer’s disease: Noticeable differences in the lipid peroxidation level and in antioxidant defense. Neurochem Res 2001,26(4),353-361
4. Siems W.; Grune T.; Lipid peroxidation measurements – methodological approaches and clinical importance Free radicals and diseases: Gene expression cellular metabolism and pathophysiology Grune T 2005,Vol. 367,11-22
5. Doorn J.A.; Petersen D.R.; Covalent modification of amino acid nucleophiles by the lipid peroxidation products 4-hydroxy-2-nonenal and 4-oxo-2-nonenal. Chem Res Toxicol 2002,15(11),1445-1450
Cited by
32 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献