Affiliation:
1. Department of Immunology, Shiraz University of Medical Sciences, Shiraz,Iran
Abstract
Background:
Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of
premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the
context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these
cells in the context of PCOS.
Objective:
To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using
two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS).
Methods:
In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western
blot technique was used to confirm the identified proteins.
Results:
Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator
complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular
metabolism especially glycolysis and ferroptosis pathway.
Conclusion:
These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.
Funder
Shiraz University of Medical Science
Publisher
Bentham Science Publishers Ltd.
Subject
Immunology and Allergy,Endocrinology, Diabetes and Metabolism
Cited by
10 articles.
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