Comprehensive evaluation of enzymatic proteolysis for amlyoid reduction in macular amyloidosis by in vitro, in vivo and in silico methods

Author:

Neve Vrushali1ORCID,Bhalchim Vrushali1,Karwa Pawan Nandkishor1ORCID,Sabu Ajay1,Runje Swapnil1

Affiliation:

1. Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India

Abstract

Skin diseases represent a significant burden on global healthcare systems, with protein deposition playing a pivotal role in their pathogenesis, including conditions such as macular amyloidosis. This study aimed to assess the proteolytic activity of the enzyme Papain on deposited protein, offering insights into potential therapeutic strategies. Using a chicken model, the deposition of protein, a key component in skin diseases, was observed predominantly in the ileum. The dose in different concentrations was given to the ileum and observation was taken on alternative days.Dose of complex protein (albumin, beta lactalbumin, alpha-lactalbumin, Immunoglobulin 500mg/ml and 750mg /ml given respectively. Treatment with Papain demonstrated its ability to effectively break down the deposited protein. In invivo method the designated area of the animal's skin where the induction was occur to ensure proper application and observation were Shaved and cleaned. One vehicle control group, one disease group and one test group. The dosing was done by subcutaneous route. Dose of complex protein (albumin, beta lactalbumin, alpha-lactalbumin, Immunoglobulin) 500mg/ml and 750mg /ml were given . For Papaya Extract Dose of 500mg/ml and 750mg /ml were given to animals. In cheken ilium 5days after dosing, length of patch were observed for five days. In wistar rat after 4days the rats seem to develop hyper pigmented patches which were brown in color in disease group. Then the groups of Animal were injected with papain by SC route. Daily interval observations were done. On the 4th day treatment spot becomes reduced and it disappeared slowly. In silico score observed upto -8 which indicate positive binding affinity.These findings underscore the potential of Papain as a promising candidate for the management of protein-related skin disorders, highlighting the importance of enzymatic intervention in mitigating their pathological manifestations.

Publisher

IP Innovative Publication Pvt Ltd

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3