Finding significant pathogens in blood cultures in children: Should we set the timer to 36 hours?

Abstract

Background: Knowledge of time to positivity (TTP) for blood cultures is useful to assess timing of discontinuation of empiric antimicrobials for suspected bacteremia with no focus. Methods: An audit of positive blood cultures from the Children's Hospital of Eastern Ontario (CHEO) from November 1, 2019, to October 31, 2020, was performed to determine TTP defined as the start of incubation to a positive signal from automated incubators. Results: Three hundred seventy-six positive blood cultures were identified from 248 patients (average age: 6.27 ± 6.24 years). Of these, 247 isolates were speciated; 90 (36.4%) were definitive/probable (DP) pathogens (median TTP 12.75 hours) and 157 (63.6%) possible/probable (PP) contaminants (median TTP of 24.08 hours). At each time point, the adjusted rate of positive blood culture was significantly higher for DP pathogens compared to PP contaminants (hazard ratio [HR]: 1.80 [1.37, 2.36]) and for children ≤27 days old compared to the oldest age group (HR: 1.94 [1.19, 3.17]). By 36 hours, the proportion of positive cultures was significantly higher in the youngest age group (≤27 days) compared with the 3–11 years old age group 91.7% (68.6%, 97.8%) versus 58.2% (46.91%, 68.06%). Conclusion: Across all ages, the TTP was significantly shorter for blood cultures with DP pathogens compared to those with PP contaminants (HR 1.80 [1.37, 2.36]). In newborns, 90% of blood cultures are positive by 36 hours supporting this re-assessment time for empiric antimicrobials. TTP was longer in children ≥12 months, possibly related to other factors such as blood culture volume.