Muscle memory in humans: evidence for myonuclear permanence and long‐term transcriptional regulation after strength training

Author:

Cumming Kristoffer Toldnes12ORCID,Reitzner Stefan Markus34ORCID,Hanslien Marit1,Skilnand Kenneth1,Seynnes Olivier R.1,Horwath Oscar5ORCID,Psilander Niklas5ORCID,Sundberg Carl Johan36,Raastad Truls1

Affiliation:

1. Department of Physical Performance Norwegian School of Sport Sciences Oslo Norway

2. Faculty of Health, Welfare and Organisation Østfold University College Fredrikstad Norway

3. Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden

4. Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden

5. Department of Physiology, Nutrition and Biomechanics, Åstrand Laboratory Swedish School of Sport and Health Sciences Stockholm Sweden

6. Department of Learning, Informatics, Management and Ethics Karolinska Institutet Stockholm Sweden

Abstract

AbstractThe objective of this work was to investigate myonuclear permanence and transcriptional regulation as mechanisms for cellular muscle memory after strength training in humans. Twelve untrained men and women performed 10 weeks of unilateral elbow‐flexor strength training followed by 16 weeks of de‐training. Thereafter, 10 weeks’ re‐training was conducted with both arms: the previously trained arm and the contralateral untrained control arm. Muscle biopsies were taken from the trained arm before and after both training periods and from the control arm before and after re‐training. Muscle biopsies were analysed for fibre cross‐sectional area (fCSA), myonuclei and global transcriptomics (RNA sequencing). During the first training period, myonuclei increased in type 1 (13 ± 17%) and type 2 (33 ± 23%) fibres together with a 30 ± 43% non‐significant increase in mixed fibre fCSA (P = 0.069). Following de‐training, fCSA decreased in both fibre types, whereas myonuclei were maintained, resulting in 33% higher myonuclear number in previously trained vs. control muscle in type 2 fibres. Furthermore, in the previously trained muscle, three differentially expressed genes (DEGs; EGR1, MYL5 and COL1A1) were observed. Following re‐training, the previously trained muscle showed larger type 2 fCSA compared to the control (P = 0.035). However, delta change in type 2 fCSA was not different between muscles. Gene expression was more dramatically changed in the control arm (1338 DEGs) than in the previously trained arm (822 DEGs). The sustained higher number of myonuclei in the previously trained muscle confirms myonuclear accretion and permanence in humans. Nevertheless, because of the unclear effect on the subsequent hypertrophy with re‐training, the physiological benefit remains to be determined. imageKey points Muscle memory is a cellular mechanism that describes the capacity of skeletal muscle fibres to respond differently to training stimuli if the stimuli have been previously encountered. This study overcomes past methodological limitations related to the choice of muscles and analytical procedures. We show that myonuclear number is increased after strength training and maintained during de‐training. Increased myonuclear number and differentially expressed genes related to muscle performance and development in the previously trained muscle did not translate into a clearly superior responses during re‐training. Because of the unclear effect on the subsequent hypertrophy and muscle strength gain with re‐training, the physiological benefit remains to be determined.

Funder

Centrum för idrottsforskning

Publisher

Wiley

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