Deductive approach to redox titration and kinetic-spectrophotometric methods for quantitative determination of ampicillin

Abstract

The article considers the search for new analytical reactions that can be the basis of quantitative analytical determinations of penicillins. The optimal conditions for these reactions were determined. We developed two unified procedures have been and established the possibility of quantitative determination by the methods of kinetic-spectrophotometry and redox titration of ampicillin in a pure substance and drug by using potassium caroate. The scheme of the chemical transformation of ampicillin with the reaction of potassium caroate was proposed. The kinetics of the conjugated reactions of S-oxidation and perhydrolysis of ampicillin with potassium caroate in alkaline medium was studied by an increase in the light absorbance of the reaction product at 290 nm. The appearance of a new wave in kinetic-spectrophotometry created the possibility of developing a new procedure for the quantitative determination of ampicillin. The reaction rate was monitored spectrally and displayed in real time. A differential variation of the tangent method was used to process the kinetic data.